These technologies yield reads around 150 nucleotides for Illumina and 400nt for pyrosequencing45. This is much shorter than the 1kB reads obtainable with the latest Sanger sequencing technologies. However the throughputs are much higher40: 2.5 to 12.5 Gigabases per hour for Illumina and 30 Megabases per hour for pyrosequencing. Costs are also quite low: $0.07 and $10 per Megabase for Illumina and pyrosequencing respectively. The per-base sequencing accuracies are also quite high, up to 99.9% for both Illumina46 and pyrosequencing40. A summary of the key characteristics for various sequencing technologies can be found in Table 1.1. The lower cost and higher throughput has made the Illumina sequencing technology the dominant one. The company estimates that 90% of the world’s sequencing data was generated with Illumina machines in 201547.
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